zhanusova 13.12.2022

Overview of recent publications

Prion Laboratory is mainly focused on the basic research connected to prions, prion diseases, other proteinopathies and the possibilities of their diagnosis.


Soukup J, Kostelanska M, Kereïche S, Hujacová A, Pavelcová M, Petrák J, Kubala Havrdová E, Holada K. Flow Cytometry Analysis of Blood Large Extracellular Vesicles in Patients with Multiple Sclerosis Experiencing Relapse of the Disease. Clin Med. 2022 May 17;11(10):2832. doi: 10.3390/jcm11102832. [pubmed]

Kostelanska M, Holada K. Prion Strains Differ in Susceptibility to Photodynamic Oxidation. Molecules 2022;27(3):611. doi: 10.3390/molecules27030611. [pubmed]


Pekarkova K, Soukup J, Kostelanska M, Sirc J, Stranak Z, Holada K. Cord Blood Extracellular Vesicles Analyzed by Flow Cytometry with Thresholding Using 405 nm or 488 nm Laser Leads to Concurrent Results. Diagnostics (Basel). 2021 Jul 22;11(8):1320. doi: 10.3390/diagnostics11081320. [pubmed]

Hujacova A, Sirc J, Pekarkova K, Brozova T, Kostelanska M, Soukup J, Mosko T, Holada K, Stranak Z. Large Platelet and Endothelial Extracellular Vesicles in Cord Blood of Preterm Newborns: Correlation with the Presence of Hemolysis. Diagnostics (Basel). 2021 Jul 22;11(8):1316. doi: 10.3390/diagnostics11081316. [pubmed]

Galuskova S, Mosko T, Dusek P, Matej R, Holada K. New possibilities of laboratory diagnostics of diseases associated with amyloid formation. Cesk Slov Neurol N. 2021;84/117(4):334–340. [abstract]

Mosko T, Galuskova S, Matej R, Bruzova M, Holada K. Detection of Prions in Brain Homogenates and CSF Samples Using a Second-Generation RT-QuIC Assay: A Useful Tool for Retrospective Analysis of Archived Samples. Pathogens. 2021 Jun 13;10(6):750. doi: 10.3390/pathogens10060750. [pubmed]


Jankovska E, Lipcseyova D, Svrdlikova M, Pavelcova M, Kubala Havrdova E, Holada K, Petrak J. Quantitative proteomic analysis of cerebrospinal fluid of women newly diagnosed with multiple sclerosis. Int J Neurosci. 2020 Oct 29;1-11. doi: 10.1080/00207454.2020.1837801. [pubmed]

Hujacova A, Brozova T, Mosko T, Kostelanska M, Stranak Z, Holada K. Platelet Extracellular Vesicles in Cord Blood of Term and Preterm Newborns Assayed by Flow Cytometry: the Effect of Delay in Sample Preparation and of Sample Freezing. Folia Biol (Praha). 2020;66(5-6):204-211. [pubmed]


Fremuntova Z, Mosko T, Soukup J, Kucerova J, Kostelanska M, Hanusova ZB, Filipova M, Cervenakova L, Holada K. Changes in cellular prion protein expression, processing and localization during differentiation of the neuronal cell line CAD 5. Biol Cell. 2020;112(1):1–21. [pubmed]

Kostelanska M, Freisleben J, Backovska Hanusova Z, Mosko T, Vik R, Moravcova D, Hamacek A, Mosinger J, Holada K. Optimization of the photodynamic inactivation of prions by a phthalocyanine photosensitizer: the crucial involvement of singlet oxygen. J Biophotonics. 2019;e201800340. [pubmed]

Jankovska E, Svitek M, Holada K, Petrak J. Affinity depletion versus relative protein enrichment: a side-by-side comparison of two major strategies for increasing human cerebrospinal fluid proteome coverage. Clin Proteom. 2019;16:9. [pubmed]


Filipova M, Elhelu OK, De Paoli SH, Fremuntova Z, Mosko T, Cmarko D, Simak J, Holada K. An effective "three-in-one" screening assay for testing drug and nanoparticle toxicity in human endothelial cells. PLoS One. 2018;13(10):e020655. [pubmed]

Julak J, Hujacova A, Scholtz V, Khun J, Holada K. Contribution to the Chemistry of Plasma-Activated Water. Plasma Phys Rep. 2018;44(1):125–136. [abstract]


Hanusova Z, Mosko T, Marej R, Holada K. Precision in the design of an experimental study deflects the significance of proteinase-activated receptor 2 expression in scrapie-inoculated mice. J Gen Virol. 2017;98(6):1563–1569. [pubmed]

Safarik M, Mosko T, Zawada Z, Safarikova E, Dracinsky M, Holada K, Sebestik J. Reactivity of 9-aminoacridine drug quinacrine with glutathione limits its antiprion activity. Chem Biol Drug Des. 2017;89(6):932–942. [pubmed]


Filipova M, Rusina R, Holada K. Nanoparticle-based Drug Delivery Systems Cros­s­ing Blood-brain Bar­rier – Hope for Future Treatment of Neurodegenerative Disorders? Cesk Slov Neurol N. 2016;79(2):160–167. [abstract]


Jindrova Z, Hanusova Z, Holada K. Protease-Activated Receptors: Activation, Inhibition and Pharmaceutical Relevance. Chemicke listy. 2015;109(7):507–514. [abstract]

Hanusova Z, Kucerova J, Filipova M, Jindrova Z, Holada K. Prion Protein, its Role in Cellular Proliferation, Differentiation and Nervous System Development. Cesk Slov Neurol N. 2015;78(4):406–412. [abstract]

Glier H, Simak J, Panigaj M, Gelderman MP, Vostal JG, Holada K. Expression of the cellular prion protein affects posttransfusion recovery and survival of red blood cells in mice. Transfusion. 2015;55(11):2590–2596. [pubmed]

Matej R, Rohan Z, Holada K, Olejar T. The Contribution of Proteinase-Activated Receptors to Intracellular Signaling, Transcellular Transport and Autophagy in Alzheimer´s Disease. Curr Alzheimer Res. 2015;12(1):2–12. [pubmed]


Orecna M, De Paoli SH, Janouskova O, Tegegn TZ, Filipova M, Bonevich JE, Holada K, Simak J. Toxicity of carboxylated carbon nanotubes in endothelial cells is attenuated by stimulation of the autophagic flux with the release of nanomaterial in autophagic vesicles. Nanomedicine. 2014; 10(5):939–948. [pubmed]

De Paoli SH, Diduch LL, Tegegn TZ, Orecna M, Strader MB, Karnaukhova E, Bonevich JE, Holada K, Simak J. The effect of protein corona composition on the interaction of carbon nanotubes with human blood platelets. Biomaterials. 2014; 35(24):6182–6194. [pubmed]


Dvorakova E, Vranac T, Janouskova O, Cernilec M, Koren S, Lukan A, Novakova J, Matej R, Holada K, Curin Serbec V. Detection of the GPI-anchorless prion protein fragment PrP226* in human brain. BMC Neurol. 2013;13:126. [pubmed]

Zawada Z, Safarik M, Dvorakova E, Janouskova O, Brezinova A, Stibor I, Holada K, Bour P, Hlavacek J, Sebestik J. Quinacrine reactivity with prion proteins and prion-derived peptides. Amino Acids. 2013;44(5):1279–1292. [pubmed]

Rusina R, Fiala J, Holada K, Matejckova M, Novakova J, Ampapa R, Koukolik F, Matej R. Gerstmann-Sträussler-Scheinker syndrome with the P102L pathogenic mutation presenting as familial Creutzfeldt-Jakob disease: a case report and review of the literature. Neurocase. 2013;19(1):41–53. [pubmed]


Janouskova O, Rakusan J, Karaskova M, Holada K. Photodynamic inactivation of prions by disulfonated hydroxyaluminium phthalocyanine. J Gen Virol. 2012;93:2512–2517. [pubmed]

Matej R, Olejar T, Janouskova O, Holada K. Deletion of protease-activated receptor 2 prolongs survival of scrapie-inoculated mice. J Gen Virol. 2012;93:2057–2061. [pubmed]

Glier H, Holada K. Blood storage affects the detection of cellular prion protein on peripheral blood leukocytes and circulating dendritic cells in part by promoting platelet satellitism. J Immunol Methods. 2012;380:65–72. [pubmed]

Julak J, Scholtz V, Kotucova S, Janouskova O. The persistent microbicidal effect in water exposed to the corona discharge. Phys Med. 2012 Jul;28(3):230–239. [pubmed]

Dvorakova E, Holada K. Conformation Specific Antibodies and Diagnosis of Prion Diseases. Cesk Slov Neurol N. 2012;75(3):283–290. [abstract]


Dvorakova E, Prouza M, Janouskova O, Panigaj M, Holada K. Development of monoclonal antibodies specific for glycated prion protein. J Toxicol Environ Health A. 2011;74:1469–1475. [pubmed]

Panigaj M, Glier H, Wildova M, Holada K. Expression of prion protein in mouse erythroid progenitors and differentiating murine erythroleukemia cells. PLoS One. 2011;6(9):e24599. [pubmed]

De Paoli Lacerda SH, Semberova J, Holada K, Simakova O, Hudson SD, Simak J. Carbon Nanotubes Activate Store-Operated Calcium Entry in Human Blood Platelets. ACS Nano 2011;5:5808–5813. [pubmed]

Panigaj M, Brouckova A, Glierova H, Dvorakova E, Simak J, Vostal JG, Holada K. Underestimation of the expression of cellular prion protein on human red blood cells. Transfusion. 2011;51(5):1012–1021. [pubmed]

Julak J, Janouskova O, Scholtz V, Holada K. Inactivation of prions using electrical DC discharges at atmospheric pressure and ambient temperature. Plasma Process Polym. 2011; 8(4): 316–323. [abstract]


Hobzova K, Janouskova O. Cell Cultures for the Study of Prion Diseases. Cesk Slov Neurol N. 2010;73(4):379–386. [abstract]


Brouckova A, Holada K. Cellular prion protein in blood platelets associates with both lipid rafts and the cytoskeleton. Thromb Haemost. 2009;102(5): 966–974. [pubmed]

Semberova J, De Paoli Lacerda SH, Simakova O, Holada K, Gelderman MP, Simak J. Carbon nanotubes activate blood platelets by inducing extracellular Ca2+ influx sensitive to calcium entry inhibitors. Nano Lett. 2009 Sep;9(9):3312–3317. [pubmed]


Zivny JH, Gelderman MP, Xu F, Piper J, Holada K, Simak J, Vostal JG. Reduced erythroid cell and erythropoietin production in response to acute anemia in prion protein-deficient (Prnp-/-) mice. Blood Cells Mol Dis. 2008 May-Jun;40(3):302–307. [pubmed]


Holada K, Simak J, Brown P, Vostal JG. Divergent expression of cellular prion protein on blood cells of human and nonhuman primates. Transfusion. 2007 Dec;47(12):2223–2232. [pubmed]

Pavlicek A, Bednarova L, Holada K. Production, purification and oxidative folding of the mouse recombinant prion protein. Folia Microbiol. 2007;52(4):391–397. [pubmed]


Holada K, Glierova H, Simak J, Vostal JG. Expression of cellular prion protein on platelets from patients with gray platelet or Hermansky-Pudlak syndrome and the protein’s association with alpha-granules. Haematologica. 2006 Aug;91(8):1126–1129. [pubmed]
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